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QIAseq miRNA Library Kit
MicroRNA (miRNA) Expression Plasmids | OriGene
RNA from the cells was extracted using the miRNeasy kit Qiagen according to manufacturer's instructions. To determine the impact of backbone chemistry on molecular beacon sensitivity, verification and quantification assays were performed with the synthetic targets over a concentration range of nM. The molecular beacons with the greatest sensitivity i. Mature molecular beacon specificity was tested in hybridization assays that included synthetic oligos designed to block oligo blockers, nM; Table 1 binding of molecular beacons to their target mature or pre-miRNAs.
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These findings have introduced some degree of confusion in the miRNA nomenclature. To avoid further confusion and ambiguity by prematurely presuming expression levels and biological functions for a specific miRNA strand, miRBase http: Notably, the minor miRNA species, be they 5p or 3p, are evolutionarily conserved in the seed sequences signifying biological significance [ 5 ],[ 7 ]-[ 9 ]. Indeed, miR, miR, miR and miRa and miR, to name just a few, have been shown to be co-expressed in pairs in breast, thyroid, colorectal, lung and liver cancers, respectively [ 10 ]-[ 14 ].